• 靶点：
• DAPK3

• 简介：
• >>Autophagy - animal;>>Pathways in cancer;>>Bladder cancer

• 基因名称：
• DAPK3

• 蛋白名称：
• Death-associated protein kinase 3

• Human Gene Id：
• 1613

• Human Swiss Prot No：
• O43293

• Mouse Gene Id：
• 13144

• Mouse Swiss Prot No：
• O54784

• Rat Gene Id：
• 64391

• Rat Swiss Prot No：
• O88764

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human DAPK3 around the phosphorylation site of Thr265. AA range:241-290

• 特异性：
• Phospho-DAPK3 (T265) Polyclonal Antibody detects endogenous levels of DAPK3 protein only when phosphorylated at T265.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• DAPK3;ZIPK;Death-associated protein kinase 3;DAP kinase 3;DAP-like kinase;Dlk;MYPT1 kinase;Zipper-interacting protein kinase;ZIP-kinase

• 实测条带：
• 52kD

• 背景：
• Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008],

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,function:Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.,subunit:Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L.,

• 细胞定位：
• Nucleus . Cytoplasm . Predominantly localizes to the cytoplasm but can shuttle between the nucleus and cytoplasm; cytoplasmic localization is promoted by phosphorylation at Thr-299 and involves Rho/Rock signaling. .; [Isoform 1]: Nucleus . Cytoplasm .; [Isoform 2]: Nucleus . Cytoplasm .

• 组织表达：
• Widely expressed. Isoform 1 and isoform 2 are expressed in the bladder smooth muscle.

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