• 靶点：
• CD31

• 简介：
• >>Cell adhesion molecules;>>Leukocyte transendothelial migration;>>Malaria;>>Fluid shear stress and atherosclerosis

• 基因名称：
• PECAM1

• 蛋白名称：
• Platelet endothelial cell adhesion molecule

• Human Gene Id：
• 5175

• Human Swiss Prot No：
• P16284

• Mouse Gene Id：
• 18613

• Mouse Swiss Prot No：
• Q08481

• 免疫原：
• The antiserum was produced against synthesized peptide derived from human PECAM-1 around the phosphorylation site of Tyr713. AA range:686-735

• 特异性：
• Phospho-CD31 (Y713) Polyclonal Antibody detects endogenous levels of CD31 protein only when phosphorylated at Y713.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PECAM1;Platelet endothelial cell adhesion molecule;PECAM-1;EndoCAM;GPIIA';PECA1;CD antigen CD31

• 实测条带：
• 150kD

• 背景：
• The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010],

• 功能：
• function:This protein is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.,online information:CD31 entry,online information:PECAM-1,online information:The Singapore human mutation and polymorphism database,PTM:Phosphorylated on Ser and Tyr residues after cellular activation.,similarity:Contains 6 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Long isoform predominates all tissues examined, isoform Delta12 was detected only in trachea and isoform Delta14-15 only in lung, isoform Delta14 was detected in all tissues examined with the strongest expression in heart.,

• 细胞定位：
• Cell membrane ; Single-pass type I membrane protein . Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation. .; [Isoform Long]: Cell membrane ; Single-pass type I membrane protein . Membrane raft . Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. .; [Isoform Delta15]: Cell junction . Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

• 组织表达：
• Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleuk

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