Eps15 (phospho Tyr849) Polyclonal Antibody
- 货号：YP0469
- 应用：WB;ELISA
- 种属：Human;Mouse;Rat
- 蛋白名称：
- Epidermal growth factor receptor substrate 15
- 免疫原：
- Synthesized phospho-peptide around the phosphorylation site of human Eps15 (phospho Tyr849)
- 特异性：
- Phospho-Eps15 (Y849) Polyclonal Antibody detects endogenous levels of Eps15 protein only when phosphorylated at Y849.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- EPS15;AF1P;Epidermal growth factor receptor substrate 15;Protein Eps15;Protein AF-1p
- 背景：
- This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2009],
- 功能：
- disease:A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with MLL/HRX. The result is a rogue activator protein.,domain:The EH domain interacts with Asn-Pro-Phe (NPF) motifs of target proteins.,function:Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits.,PTM:Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.,similarity:Contains 2 EF-hand dom
- 细胞定位：
- Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes. .; [Isoform 2]: Early endosome membrane ; Peripheral membrane protein ; Cytoplasmic side . Colocalizes with HGS on bilayered clathrin coats on endosomes.
- 组织表达：
- Ubiquitously expressed.
- Western Blot analysis of JK cells using Phospho-Eps15 (Y849) Polyclonal Antibody
