MEK-3 (phospho Ser218) Polyclonal Antibody
- 货号:YP0170
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 靶点:
- MEK-3
- 简介:
- >>MAPK signaling pathway;>>Rap1 signaling pathway;>>Cellular senescence;>>Toll-like receptor signaling pathway;>>Fc epsilon RI signaling pathway;>>TNF signaling pathway;>>Thermogenesis;>>Inflammatory mediator regulation of TRP channels;>>GnRH signaling pathway;>>Growth hormone synthesis, secretion and action;>>Alcoholic liver disease;>>Amyotrophic lateral sclerosis;>>Pathways of neurodegeneration - multiple diseases;>>Salmonella infection;>>Yersinia infection;>>Toxoplasmosis;>>Hepatitis B;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer;>>Lipid and atherosclerosis
- 基因名称:
- MAP2K3
- 蛋白名称:
- Dual specificity mitogen-activated protein kinase kinase 3
- Human Gene Id:
- 5606
- Human Swiss Prot No:
- P46734
- Mouse Gene Id:
- 26397
- Mouse Swiss Prot No:
- O09110
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human MKK3 around the phosphorylation site of Ser189. AA range:173-222
- 特异性:
- Phospho-MEK-3 (S189) Polyclonal Antibody detects endogenous levels of MEK-3 protein only when phosphorylated at S189.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- MAP2K3;MEK3;MKK3;PRKMK3;SKK2;Dual specificity mitogen-activated protein kinase kinase 3;MAP kinase kinase 3;MAPKK 3;MAPK/ERK kinase 3;MEK 3;Stress-activated protein kinase kinase 2;SAPK kinase 2;SAPKK-2;SAPKK2
- 实测条带:
- 39kD
- 背景:
- The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:Defects in MAP2K3 may be involved in colon cancer.,enzyme regulation:Activated by dual phosphorylation on Ser-218 and Thr-222.,function:Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.,PTM:Autophosphorylated.,PTM:Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.,PTM:Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Binds to
- 细胞定位:
- nucleoplasm,cytoplasm,cytosol,membrane,
- 组织表达:
- Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
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- Antibody-FAQs
- 产品图片
- Western Blot analysis of various cells using Phospho-MEK-3 (S218) Polyclonal Antibody
- Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using MKK3 (Phospho-Ser189) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from MDA-MB-435 cells, using MKK3 (Phospho-Ser189) Antibody. The lane on the left is blocked with the phospho peptide.