Histone H3 (phospho Thr3) Polyclonal Antibody
- 货号:YP0131
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat
- 靶点:
- Histone H3
- 简介:
- >>Neutrophil extracellular trap formation;>>Alcoholism;>>Shigellosis;>>Transcriptional misregulation in cancer;>>Systemic lupus erythematosus
- 基因名称:
- HIST1H3A
- 蛋白名称:
- Histone H3.1
- Human Gene Id:
- 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
- Human Swiss Prot No:
- P68431
- Mouse Gene Id:
- 319152
- Mouse Swiss Prot No:
- P68433
- Rat Gene Id:
- 291159
- Rat Swiss Prot No:
- Q6LED0
- 免疫原:
- The antiserum was produced against synthesized peptide derived from human Histone H3 around the phosphorylation site of Thr3. AA range:1-50
- 特异性:
- Phospho-Histone H3 (T3) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at T3.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 浓度:
- 1 mg/ml
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- HIST1H3A;H3FA;HIST1H3B;H3FL;HIST1H3C;H3FC;HIST1H3D;H3FB;HIST1H3E;H3FD;HIST1H3F;H3FI;HIST1H3G;H3FH;HIST1H3H;H3FK;HIST1H3I;H3FF;HIST1H3J;H3FJ;Histone H3.1;Histone H3/a;Histone H3/b;Histone H3/c;Histone H3/d;Histone H3
- 实测条带:
- 20kD
- 背景:
- Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],
- 功能:
- caution:Was originally (PubMed:2587222) thought to originate from mouse.,developmental stage:Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.,function:Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.,mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589,miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).,PTM:Acetylation is generally l
- 细胞定位:
- Nucleus. Chromosome.
- 组织表达:
- Blood,Epithelium,Kidney,Lung,Ovary,Spleen,Uterus,
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- Antibody-FAQs
- 产品图片
- Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Histone H3 (Phospho-Thr3) Antibody. The picture on the right is blocked with the phospho peptide.
- Western blot analysis of lysates from HUVEC cells treated with Serum 20% 30', using Histone H3 (Phospho-Thr3) Antibody. The lane on the right is blocked with the phospho peptide.