Bcr (phospho Tyr177) Polyclonal Antibody
- 货号：YP0036
- 应用：WB;ELISA
- 种属：Human;Mouse
- 简介：
- >>Pathways in cancer;>>Chronic myeloid leukemia
- 蛋白名称：
- Breakpoint cluster region protein
- 免疫原：
- The antiserum was produced against synthesized peptide derived from human Bcr around the phosphorylation site of Tyr177. AA range:144-193
- 特异性：
- Phospho-Bcr (Y177) Polyclonal Antibody detects endogenous levels of Bcr protein only when phosphorylated at Y177.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- BCR;BCR1;D22S11;Breakpoint cluster region protein;Renal carcinoma antigen NY-REN-26
- 背景：
- A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,disease:A chromosomal aberration involving BCR is a cause of chronic myeloid leukemia (CML) [MIM:608232]. Translocation t(9;22)(q34;q11) with ABL1. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).,domain:The DH domain is involved in interaction with CCPG1.,domain:The region involved in binding to ABL1 SH2-domain is rich in serine residues and needs to be Ser/Thr phosphorylated prior to SH2 binding. This region is essential for the activation of the ABL1 tyrosine kinase and transforming potential of the chimeric BCR-ABL oncogene.,function:GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.,PTM:Autophosphorylated.,similarity:Contains 1 C2 domai
- 细胞定位：
- Cell junction, synapse, postsynaptic density . Cell projection, dendritic spine . Cell projection, axon . Cell junction, synapse .
- 组织表达：
- Brain,Epithelium,Platelet,Renal cell carcinoma,T-cell,
- Western Blot analysis of various cells using Phospho-Bcr (Y177) Polyclonal Antibody
- Western blot analysis of lysates from K562 cells, using Bcr (Phospho-Tyr177) Antibody. The lane on the right is blocked with the phospho peptide.
