• 靶点：
• HSC70

• 简介：
• >>Spliceosome;>>MAPK signaling pathway;>>Protein processing in endoplasmic reticulum;>>Endocytosis;>>Longevity regulating pathway - multiple species;>>Antigen processing and presentation;>>Estrogen signaling pathway;>>Prion disease;>>Legionellosis;>>Toxoplasmosis;>>Measles;>>Lipid and atherosclerosis

• 基因名称：
• HSPA8

• 蛋白名称：
• Heat shock cognate 71 kDa protein

• Human Gene Id：
• 3312

• Human Swiss Prot No：
• P11142

• Mouse Swiss Prot No：
• P63017

• Rat Swiss Prot No：
• P63018

• 免疫原：
• Synthesized peptide derived from the Internal region of human HSC 70. AA range: 588-638

• 特异性：
• The antibody detects endogenous HSC 70 protein.

• 组成：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• 来源：
• Polyclonal, Rabbit,IgG

• 稀释：
• WB 1:500 - 1:2000.IHC:1:50-300.. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HSPA8;HSC70;HSP73;HSPA10;Heat shock cognate 71 kDa protein;Heat shock 70 kDa protein 8

• 实测条带：
• 70-72kD

• 背景：
• This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011],

• 功能：
• function:Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co-chaperones.,induction:Constitutively synthesized.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the heat shock protein 70 family.,subcellular location:Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:Interacts with HSPH1/HSP105. Interacts with IRAK1BP1 (By similarity). Interacts with PACRG and TSC2. Interacts with SV40 VP1.,tissue specificity:Ubiquitous.,

• 细胞定位：
• Cytoplasm. Melanosome. Nucleus, nucleolus. Cell membrane. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Translocates rapidly from the cytoplasm to the nuclei, and especially to the nucleoli, upon heat shock.

• 组织表达：
• Ubiquitous.

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