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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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APC4 Polyclonal Antibody
- 货号:YN1797
- 应用:WB;ELISA
- 种属:Human;Mouse
- 简介:
- >>Cell cycle;>>Oocyte meiosis;>>Ubiquitin mediated proteolysis;>>Progesterone-mediated oocyte maturation;>>Human T-cell leukemia virus 1 infection
- 蛋白名称:
- Anaphase-promoting complex subunit 4 (APC4) (Cyclosome subunit 4)
- 免疫原:
- Synthesized peptide derived from part region of human protein
- 特异性:
- APC4 Polyclonal Antibody detects endogenous levels of protein.
- 组成:
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 来源:
- Polyclonal, Rabbit,IgG
- 稀释:
- WB 1:500-2000 ELISA 1:5000-20000
- 纯化工艺:
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitor, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eight subunits. The composition of the APC is highly conserved in organisms from yeast to humans. The exact function of this gene product is not known. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013],
- 功能:
- function:Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle.,pathway:Protein modification; protein ubiquitination.,similarity:Belongs to the APC4 family.,subunit:The APC/C is composed of at least 11 subunits.,
- 组织表达:
- Amygdala,Placenta,Testis,