GNA13 Polyclonal Antibody
- 货号：YN0753
- 应用：WB;ELISA
- 种属：Human;Mouse
- 简介：
- >>cGMP-PKG signaling pathway;>>Sphingolipid signaling pathway;>>Phospholipase D signaling pathway;>>Vascular smooth muscle contraction;>>Apelin signaling pathway;>>Platelet activation;>>Long-term depression;>>Regulation of actin cytoskeleton;>>Parathyroid hormone synthesis, secretion and action;>>Pathogenic Escherichia coli infection;>>Human cytomegalovirus infection;>>Pathways in cancer
- 蛋白名称：
- Guanine nucleotide-binding protein subunit alpha-13 (G alpha-13) (G-protein subunit alpha-13)
- 免疫原：
- Synthesized peptide derived from part region of human protein
- 特异性：
- GNA13 Polyclonal Antibody detects endogenous levels of protein.
- 组成：
- Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
- 来源：
- Polyclonal, Rabbit,IgG
- 稀释：
- WB 1:500-2000 ELISA 1:5000-20000
- 纯化工艺：
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景：
- function:Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.,PTM:Palmitoylation is critical for proper membrane localization and signaling.,PTM:Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.,similarity:Belongs to the G-alpha family. G(12) subfamily.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5.,
- 功能：
- function:Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.,PTM:Palmitoylation is critical for proper membrane localization and signaling.,PTM:Phosphorylation on Thr-203 by PKA destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.,similarity:Belongs to the G-alpha family. G(12) subfamily.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subunit:G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5.,
- 细胞定位：
- Cell membrane ; Lipid-anchor . Melanosome . Cytoplasm . Nucleus . Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Detected in the cytoplasm of Leydig cells and in the seminiferous epithelium, including differentiating cells from the spermatogonia to mature spermatozoa stages (PubMed:18703424). In round spermatids, also present in the nuclei (PubMed:18703424). .
- 组织表达：
- Expressed in testis, including in Leydig cells and in the seminiferous epithelium, in differentiating cells from the spermatogonia to mature spermatozoa stages and round spermatids (at protein level). Expressed in 99.2% of spermatozoa from healthy individuals, but only in 28.6% of macrocephalic spermatozoa from infertile patients (at protein level).
- Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°over night
