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- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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PAP (ABT66R) rabbit mAb
- 货号:YM7273
- 应用:IHC; ELISA
- 种属:Human;(predicted: Mouse;Rat)
- 蛋白名称:
- Prostatic Acid Phosphatase(PSAP)
- 免疫原:
- Synthesized peptide derived from human Prostatic Acid Phosphatase(PSAP) AA range:201-300
- 特异性:
- This antibody detects endogenous levels of PSAP
- 组成:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 来源:
- Monoclonal, Rabbit IgG1, Kappa
- 稀释:
- IHC 1:100-500, ELISA 1:5000-20000
- 纯化工艺:
- Recombinant Expression and Affinity purified
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- 5' NT;5' nucleotidase;5'-NT;5'-nucleotidase;Acid phosphatase prostate;ACP 3;ACP3;acpP;Ecto 5' nucleotidase;Ecto-5'-nucleotidase;PAP;PPAP_HUMAN;Prostatic acid phosphatase;Prostatic acid phosphotase;Thiamine monophosphatase;TMPase
- 功能:
- catalytic activity:A phosphate monoester + H(2)O = an alcohol + phosphate.,similarity:Belongs to the histidine acid phosphatase family.,subcellular location:Predominantly localized in the plasma membrane but also detected in intracellular vesicles.,subunit:Homodimer.,
- 细胞定位:
- [Isoform 1]: Secreted .; [Isoform 2]: Cell membrane ; Single-pass type I membrane protein . Lysosome membrane ; Single-pass type I membrane protein . Nucleus . Cytoplasm, cytosol . Appears to shuttle between the cell membrane and intracellular vesicles. Colocalizes with FLOT1 at cell membrane and in intracellular vesicles (PubMed:17638863). Colocalizes with LAMP2 on the lysosome membrane (PubMed:17897319). .
- 组织表达:
- Highly expressed in the prostate, restricted to glandular and ductal epithelial cells. Also expressed in bladder, kidney, pancreas, lung, cervix, testis and ovary. Weak expression in a subset of pancreatic islet cells, squamous epithelia, the pilosebaceous unit, colonic neuroendocrine cells and skin adnexal structures. Low expression in prostate carcinoma cells and tissues. ; [Isoform 2]: Widely expressed. Expressed in the sarcolemma of skeletal muscle.
![](/uploadfiles/PIC/ABT66R-Placenta.jpg)
- Immunohistochemical analysis of paraffin-embedded human Placenta. 1, Antibody was incubated at 4° overnight. 2, TRIS-EDTA of pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).