Vimentin (ABT281) mouse mAb (Ready to Use)
- 货号：YM6918R
- 应用：IHC
- 种属：Human;Mouse;Rat;
- 简介：
- >>Epstein-Barr virus infection;>>MicroRNAs in cancer
- 免疫原：
- Synthesized peptide derived from human Vimentin AA range: 400-466
- 特异性：
- The antibody can specifically recognize human Vimentin protein.
- 组成：
- The prediluted ready-to-use antibody is diluted in phosphate buffer saline containing stabilizing protein and 0.05% Proclin 300
- 来源：
- Mouse, Monoclonal/IgG1, kappa
- 纯化工艺：
- The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
- 背景：
- This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009],
- 功能：
- function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.,online information:Vimentin entry,PTM:One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.,sequence caution:Intron retention.,similarity:Belongs to the intermediate filament family.,subunit:Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM.,tissue specificity:Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.,
- 组织表达：
- Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
- Human endometrial adenocarcinoma tissue was stained with Anti-Vimentin (ABT281) Antibody
- Human hepatocellular carcinoma tissue was stained with Anti-Vimentin (ABT281) Antibody
- Human rectal carcinoma tissue was stained with Anti-Vimentin (ABT281) Antibody
- Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-Vimentin (YM6918), Anti-p120 (YM6086), Anti-Topoisomerase IIα (YM6914).
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit (RS0039, Immunoway).
The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (YM6918 1:200), Anti-p120 (YM6086 1:200), Anti-Topoisomerase IIα (YM6914 1:200).; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
- Fluorescence multiplex immunohistochemical analysis of Human tonsil tissue (formalin-fixed paraffin-embedded section).
Merged staining of Anti-Vimentin (YM6918), Anti-p120 (YM6086), Anti-Topoisomerase IIα (YM6914).
The immunostaining was performed on a Leica Biosystems BOND® MAX instrument with an Sextuple-Fluorescence kit (RS0039, Immunoway).
The section was incubated in 3 rounds of staining; sequentially for Anti-Vimentin (YM6918 1:200), Anti-p120 (YM6086 1:200), Anti-Topoisomerase IIα (YM6914 1:200).; each using a separate fluorescent tyramide signal amplification system. EDTA based antigen retrieval (Leica Biosystems BOND® Epitope Retrieval Solution 2, pH 9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibody from the previous round, to avoid any cross-reactivity. DAPI (dark blue) was used as a nuclear counter stain.
Microscopy and pseudocoloring of individual dyes was performed using a Slideviewer Imaging System (3D histech).
