Galectin 3 (ABT-GAL3) Mouse mAb
- 货号:YM6764
- 应用:IHC;WB;ELISA
- 种属:Human
- 免疫原:
- Synthesized peptide derived from human Galectin 3 AA range: 200-300
- 特异性:
- This antibody detects endogenous levels of human Galectin 3, TRIS-EDTA of pH9.0 was used for Heat-induced epitope retrieval (HIER)
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 来源:
- Mouse, Monoclonal/IgG2b, Kappa
- 稀释:
- IHC-p 1:50-500, WB 1:1000-2000, ELISA 1:5000-20000
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Galectin-3 (Gal-3;35 kDa lectin;Carbohydrate-binding protein 35;CBP 35;Galactose-specific lectin 3;Galactoside-binding protein;GALBP;IgE-binding protein;L-31;Laminin-binding protein;Lectin L-29;Mac-2 antigen)
- 背景:
- This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. The protein exhibits antimicrobial activity against bacteria and fungi. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Oct 2014],
- 功能:
- function:Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis.,online information:Galectin-3,similarity:Contains 1 galectin domain.,subcellular location:Cytoplasmic in adenomas and carcinomas. May be secreted by a non-classical secretory pathway and associate with the cell surface.,subunit:Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.,tissue specificity:A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.,
- 细胞定位:
- Cytoplasmic, Nuclear
- 组织表达:
- A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.
- Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-Galectin-3 antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.
Predicted band size: 26(30) kDa
Observed band size: 33 kDa
- Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Galectin-3 and anti-GAPDH antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.
Lane 1 : Galectin-3 knockout Hela cell lysate
Lane 2 : Wide type Hela cell lysate
Predicted band size: 30 kDa
Observed band size: 30 kDa
- Human ovarian carcinoma tissue was stained with Anti-Galectin 3 (ABT-GAL3) Antibody
- Human thyroid papillary carcinoma tissue was stained with Anti-Galectin 3 (ABT-GAL3) Antibody
- Human thyroid papillary carcinoma tissue was stained with Anti-Galectin 3 (ABT-GAL3) Antibody
- Human thyroid papillary carcinoma tissue was stained with Anti-Galectin 3 (ABT-GAL3) Antibody