Nuclear protein(NeuN)(PTR1373)mouse mAb
- 货号：YM4669
- 应用：WB;IHC;IF;ELISA
- 种属：Human;
- 蛋白名称：
- NeuN Neuronal Marker
- 免疫原：
- Synthesized peptide derived from human protein. AA range:200-300
- 特异性：
- This antibody detects endogenous levels of Nuclear protein(NeuN) protein.
- 组成：
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 来源：
- Monoclonal,Mouse,IgG2a,kappa
- 稀释：
- IHC 1:200-1000.WB 1:500-2000.IF 1:100-500.ELISA 1:1000-5000.
- 纯化工艺：
- The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RNA binding protein fox-1 homolog 3 (Fox-1 homolog C)
- 背景：
- This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2017]
- 细胞定位：
- Cytoplasmic, Nuclear
- Various whole cell lysates were separated by 4%-20% SDS-PAGE, and the membrane was blotted with anti-Nuclear protein(NeuN) (PTR1373) antibody. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Lane 1: MCF7
Lane 2: SH-SY5Y
Predicted band size: 34kDa
Observed band size: 40kDa
