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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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MGMT (PTR2300) mouse mAb
- 货号:YM4650
- 应用:WB;IF;ELISA
- 种属:Human;
- 蛋白名称:
- Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase)
- 免疫原:
- Synthesized peptide derived from human MGMT AA range: 1-100
- 特异性:
- This antibody detects endogenous levels of MGMT protein.
- 组成:
- PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
- 来源:
- Mouse, Monoclonal/IgG2b, kappa
- 稀释:
- WB 1:500-2000. IF 1:100-500. ELISA 1:1000-5000
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- O-6-methylguanine-DNA methyltransferase(MGMT) Homo sapiens Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015],
- 功能:
- Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
![](/uploadfiles/PIC/PTR2300-Jurkat.jpg)
- Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MGMT (PTR2300) antibody. The HRP-conjugated Goat anti-Mouse IgG(H + L) antibody was used to detect the antibody.
Lane 1: Jurkat