Pan Methylated Lysine Monoclonal Antibody(Mix)
- 货号:YM3429
- 应用:WB;IHC;IF
- 种属:Species independent
- 靶点:
- Pan Methylated Lysine
- 免疫原:
- Conjugated Protein
- 特异性:
- The antibody detects endogenous Pan Methylated Lysine protein.
- 组成:
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 来源:
- Monoclonal, Mouse
- 稀释:
- WB 1:1000-2000 IHC 1:200-500. IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 背景:
- Methylation of lysine residues is a common regulatory posttranslational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs). Two PKMT groups are recognized based on structure and catalytic mechanism: class I methyltransferases or seven β strand enzymes, and SET domain-containing class V methyltransferases. Both use the methyl donor S-adenosyl-L-methionine to methylate histone and non-histone proteins. Class I methyltransferases methylate amino acids, DNA, and RNA. Six methyl-lysine-interacting protein families are distinguished based on binding domains: mBT, PHD finger, Tudor, PWWP, WD40 repeat, and chromodomains. Many of these display differential binding preferences based on lysine methylation state. KDM1 subfamily lysine demethylases catalyze demethylation of mono- and di-methyl lysines, while 2-oxoglutarate-dependent JmjC (KDM2-7) subfamily enzymes also modify tri-methyl lysine residues.
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- Antibody-FAQs
- 产品图片
- Western blot analysis of Hela using Pan Methylated Lysine Monoclonal Antibody.
- Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using Pan Methylated Lysine Monoclonal Antibody.