CD15 Monoclonal Antibody(Q89)
- 货号:YM3105
- 应用:IHC;IF
- 种属:Human
- 简介:
- >>Mannose type O-glycan biosynthesis;>>Glycosphingolipid biosynthesis - lacto and neolacto series;>>Metabolic pathways
- 蛋白名称:
- Alpha-(1,3)-fucosyltransferase
- 免疫原:
- Synthetic Peptide of CD15
- 特异性:
- The antibody detects endogenous CD15 protein.
- 组成:
- PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
- 稀释:
- IHC 1:200 IF 1:50-200
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FUT4;ELFT;FCT3A;Alpha-(1,3)-fucosyltransferase;ELAM-1 ligand fucosyltransferase;Fucosyltransferase 4;Fucosyltransferase IV;Fuc-TIV;FucT-IV;Galactoside 3-L-fucosyltransferase
- 背景:
- The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009],
- 功能:
- caution:It is uncertain whether Met-1 or Met-126 is the initiator.,function:May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.,online information:Fucosyltransferase 4,online information:GlycoGene database,pathway:Protein modification; protein glycosylation.,similarity:Belongs to the glycosyltransferase 10 family.,subcellular location:Membrane-bound form in trans cisternae of Golgi.,
- 细胞定位:
- Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Membrane-bound form in trans cisternae of Golgi.
- 组织表达:
- [Isoform Short]: Expressed at low levels in bone marrow-derived mesenchymal stem cells. ; Expressed in cord blood immature promyelocytes and in peripheral blood myeloid and lymphoid cell populations.
- Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD15 Monoclonal Antibody(Q89) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
- Immunofluorescence analysis of Human-liver-cancer tissue. 1,CD15 Monoclonal Antibody(Q89)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
- Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using CD 15 Mouse mAb diluted at 1:500.
- Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).