• 靶点：
• Cytokeratin 8

• 基因名称：
• KRT8

• 蛋白名称：
• Keratin type II cytoskeletal 8

• Human Gene Id：
• 3856

• Human Swiss Prot No：
• P05787

• Mouse Gene Id：
• 16691

• Mouse Swiss Prot No：
• P11679

• Rat Gene Id：
• 25626

• Rat Swiss Prot No：
• Q10758

• 免疫原：
• Synthetic Peptide of CK8

• 特异性：
• The antibody detects endogenous CK8 proteins.

• 组成：
• PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

• 来源：
• Monoclonal, Mouse

• 稀释：
• IF 1:50-200 WB 1:2000-5000 IHC 1:200

• 纯化工艺：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• KRT8;CYK8;Keratin, type II cytoskeletal 8;Cytokeratin-8;CK-8;Keratin-8;K8;Type-II keratin Kb8

• 实测条带：
• 54kD

• 背景：
• keratin 8(KRT8) Homo sapiens This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],

• 功能：
• disease:Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM:215600].,function:Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.,miscellaneous:There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).,PTM:O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.,PTM:Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.,similarity:Belongs to the intermediate filament family.,subunit:Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP.,tissue spec

• 细胞定位：
• Cytoplasm . Nucleus, nucleoplasm . Nucleus matrix .

• 组织表达：
• Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.

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