• 靶点：
• AIF

• 简介：
• >>Apoptosis;>>Necroptosis

• 基因名称：
• aifm1

• Human Gene Id：
• 9131

• Human Swiss Prot No：
• O95831

• Mouse Swiss Prot No：
• Q9Z0X1

• 免疫原：
• Purified recombinant human AIF protein fragments expressed in E.coli.

• 特异性：
• This antibody detects endogenous levels of AIF and does not cross-react with related proteins.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• wb dilution 1:1000 icc dilution 1:200. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• AIFM1;AIFM1_HUMAN;Apoptosis inducing factor 1, mitochondrial;Apoptosis inducing factor;Apoptosis inducing factor, mitochondrion associated, 1;Apoptosis-inducing factor 1;CMTX4;COWCK;COXPD6;Harlequin;Hq;mAIF;MGC111425;MGC5706;mitochondrial;Neuropathy, axonal motor-sensory, with deafness and mental retardation;neuropathy, axonal, motor-sensory with deafness and mental retardation (Cowchock syndrome);PDCD 8;PDCD8;Programmed cell death 8 (apoptosis inducing factor);Programmed cell death 8;Programmed cell death 8 isoform 1;Programmed cell death 8 isoform 2;Programmed cell death 8 isoform 3;Programmed cell death protein 8;Programmed cell death protein 8 mitochondrial;Programmed cell death protein 8 mitochondrial precursor;Striatal apoptosis inducing factor.

• 实测条带：
• 67kD

• 背景：
• This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and mental retardation. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome

• 功能：
• catalytic activity:2 glutathione + protein-disulfide = glutathione disulfide + protein-dithiol.,cofactor:FAD.,function:Possesses significant protein thiol-disulfide oxidase activity.,function:Probable oxidoreductase that acts as a caspase-independent mitochondrial effector of apoptotic cell death. Extramitochondrial AIF induces nuclear chromatin condensation and large scale DNA fragmentation (in vitro). Binds to DNA in a sequence-independent manner.,similarity:Belongs to the FAD-dependent oxidoreductase family.,similarity:Contains 1 thioredoxin domain.,subcellular location:Translocated to the nucleus upon induction of apoptosis.,subunit:Interacts with XIAP.,tissue specificity:Widely expressed.,

• 细胞定位：
• Mitochondrion intermembrane space . Mitochondrion inner membrane. Cytoplasm . Nucleus . Cytoplasm, perinuclear region . Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis (PubMed:15775970). Colocalizes with EIF3G in the nucleus and perinuclear region (PubMed:17094969). .; [Isoform 3]: Mitochondrion intermembrane space . Mitochondrion inner membrane . Has a stronger membrane ancho

• 组织表达：
• Expressed in all tested tissues (PubMed:16644725). Detected in muscle and skin fibroblasts (at protein level) (PubMed:23217327). Expressed in osteoblasts (at protein level) (PubMed:28842795). ; [Isoform 3]: Brain specific. ; [Isoform 4]: Expressed in all tested tissues except brain. ; [Isoform 5]: Isoform 5 is frequently down-regulated in human cancers.

• June 19-2018
• WESTERN IMMUNOBLOTTING PROTOCOL

• June 19-2018
• IMMUNOHISTOCHEMISTRY-PARAFFIN PROTOCOL

• June 19-2018
• IMMUNOFLUORESCENCE PROTOCOL

• September 08-2020
• FLOW-CYTOMEYRT-PROTOCOL

• May 20-2022
• Cell-Based ELISA│解您多样本WB检测之困扰

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-ACETYL-PROTEIN

• July 13-2018
• CELL-BASED-ELISA-PROTOCOL-FOR-PHOSPHO-PROTEIN

• July 13-2018
• CELL-BASED-COLORIMETRIC-ELISA-PROTOCOL-FOR-TOTAL-PROTEIN

• July 13-2018
• Antibody-FAQs