• 靶点：
• eIF2α

• 简介：
• >>Autophagy - animal;>>Protein processing in endoplasmic reticulum;>>Apoptosis;>>Non-alcoholic fatty liver disease;>>Alzheimer disease;>>Parkinson disease;>>Amyotrophic lateral sclerosis;>>Prion disease;>>Pathways of neurodegeneration - multiple diseases;>>Hepatitis C;>>Measles;>>Influenza A;>>Herpes simplex virus 1 infection;>>Lipid and atherosclerosis

• 基因名称：
• eif2s1

• Human Gene Id：
• 1965

• Human Swiss Prot No：
• P05198

• Mouse Swiss Prot No：
• Q6ZWX6

• 免疫原：
• Purified recombinant human eIF2α protein fragments expressed in E.coli.

• 特异性：
• This antibody detects endogenous levels of eIF2α and does not cross-react with related proteins.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• wb dilution 1:1000 icc dilution 1:200. IF 1:50-200

• 纯化工艺：
• The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• Dmel\CG6376；Dmel_CG6376；drosE2F1；E(Sev-CycE)3A；E(var)3-93E；E2-promoter binding facto；E2F 1；E2F transcription factor 1；E2F-1；E2f-PA；E2f-PB；E2f-PC；E2F1；E2f1 E2F transcription factor 1；E2F1_HUMAN；Evar(3)164；KIAA4009；l(3)07172；l(3)j3B1；l(3)j3C2；l(3)rM729；mKIAA4009；OTTHUMP00000030661；PBR3；PRB binding protein E2F 1；PRB-binding protein E2F-1；RBAP 1；RBAP-1；RBAP1；RBBP-3；RBBP3；RBP 3；RBP3；Retinoblastoma-associated protein 1；Retinoblastoma-binding protein 3；Transcription factor E2F1.

• 实测条带：
• 38kD

• 背景：
• The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010],

• 功能：
• function:Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.,PTM:Substrate for at least 4 kinases: EIF2AK3/PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. In case of infection by vaccinia virus or rotavirus

• 细胞定位：
• Cytoplasm, Stress granule . Colocalizes with NANOS3 in the stress granules. .

• 组织表达：
• B cells,Brain,Fibroblast,Placenta,

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