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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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LRRFIP1 mouse mAb
- 货号:YM1320
- 应用:WB
- 种属:Human;Mouse
- 免疫原:
- Purified recombinant human LRRFIP1 protein fragments expressed in E.coli.
- 特异性:
- This antibody detects endogenous levels of LRRFIP1 and does not cross-react with related proteins.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- FLAP1;FLIIAP 1;GC binding factor 2;GCF2;HUFI1;Leucine rich repeat (in FLII) interacting protein 1;Leucine rich repeat flightless interacting protein 1;LRR FLII interacting protein 1;LRRFIP 1;Lrrfip1;MGC10947;MGC119738;MGC119739;NEDD8 conjugating enzyme;TAR RNA interacting protein;TAR RNA-interacting protein;Transcription factor 9 like;TRIP.
- 背景:
- developmental stage:Widely expressed in fetal tissues.,function:Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the LRRFIP family.,subunit:Interacts with FLII.,tissue specificity:Ubiquitously expressed.,
- 功能:
- developmental stage:Widely expressed in fetal tissues.,function:Transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. May control smooth muscle cells proliferation following artery injury through PDGFA repression. May also bind double-stranded RNA.,sequence caution:Contaminating sequence. Potential poly-A sequence.,similarity:Belongs to the LRRFIP family.,subunit:Interacts with FLII.,tissue specificity:Ubiquitously expressed.,
- 细胞定位:
- Nucleus. Cytoplasm.
- 组织表达:
- Ubiquitously expressed.
- Western blot detection of LRRFIP1 in Raji,C2C12,MCF7 and Jurkat cell lysates using LRRFIP1 mouse mAb (1:1000 diluted).Predicted band size:89KDa.Observed band size:160KDa.