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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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GTF2H1 mouse mAb
- 货号:YM1262
- 应用:WB;IP;ICC
- 种属:Human
- 简介:
- >>Basal transcription factors;>>Nucleotide excision repair;>>Viral carcinogenesis
- 免疫原:
- Purified recombinant human GTF2H1protein fragments expressed in E.coli.
- 特异性:
- This antibody detects endogenous levels of GTF2H1 and does not cross-react with related proteins.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- Basic transcription factor 2 62 kDa subunit;Basic transcription factor 62 kDa subunit;BTF 2;BTF2;BTF2 p62;General transcription factor IIH polypeptide 1;General transcription factor IIH subunit 1;General transcription factor IIH, polypeptide 1, 62kDa;gtf2h1;TF2H1;TF2H1_HUMAN;TFB1;TFIIH;TFIIH basal transcription factor complex p62 subunit.
- 背景:
- function:Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.,PTM:Phosphorylated.,similarity:Contains 2 BSD domains.,subunit:One of the six subunits forming the core-TFIIH basal transcription factor. Interacts with PUF60.,
- 功能:
- function:Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.,PTM:Phosphorylated.,similarity:Contains 2 BSD domains.,subunit:One of the six subunits forming the core-TFIIH basal transcription factor. Interacts with PUF60.,
- Western blot detection of GTF2H1 in Hela,Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.
- Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using GTF2H1 mouse mAb (dilution 1:50).
- Immunoprecipitation analysis of Hela cell lysates using GTF2H1 mouse mAb.