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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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DsbA-Tag mouse mAb
- 货号:YM1261
- 应用:WB
- 种属:E.coli
- 免疫原:
- Purified recombinant E.coli. DsbA protein fragments over expressed in E.coli.
- 特异性:
- This antibody detects endogenous levels of DsbA and does not cross-react with related proteins.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DSBA like thioredoxin domain containing protein.
- 背景:
- P0AEG4 periplasmic protein disulfide isomerase I(dsbA) Escherichia coli str. K-12 substr. MG1655 function:Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.,similarity:Belongs to the thioredoxin family. DsbA subfamily.,similarity:Contains 1 thioredoxin domain.,
- 功能:
- P0AEG4 periplasmic protein disulfide isomerase I(dsbA) Escherichia coli str. K-12 substr. MG1655 function:Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.,similarity:Belongs to the thioredoxin family. DsbA subfamily.,similarity:Contains 1 thioredoxin domain.,
- Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.
- Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments using DSBA mouse mAb (1:1000 diluted).