ATP-Citrate Lyase(C-term) mouse mAb
- 货号：YM1260
- 应用：WB;ICC;FC
- 种属：Human;Mouse;Monkey
- 简介：
- >>Citrate cycle (TCA cycle);>>Metabolic pathways
- 免疫原：
- Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.
- 特异性：
- This antibody detects endogenous levels of ATP-Citrate Lyase and does not cross-react with related proteins.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- wb 1:1000 icc 1:150 fcm 1:100
- 纯化工艺：
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ACL;Acly;ACLY_HUMAN;ATP citrate (pro-S) lyase;ATP citrate lyase;ATP citrate synthase;ATP-citrate (pro-S-)-lyase;ATP-citrate synthase;ATPcitrate synthase;ATPCL;Citrate cleavage enzyme;CLATP;EC 2.3.3.8;OTTHUMP00000164773.
- 背景：
- ATP citrate lyase(ACLY) Homo sapiens ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014],
- 功能：
- catalytic activity:ADP + phosphate + acetyl-CoA + oxaloacetate = ATP + citrate + CoA.,function:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.,similarity:In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.,similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.,subunit:Homotetramer.,
- 细胞定位：
- Cytoplasm, cytosol .
- 组织表达：
- Brain,Epithelium,Hippocampus,Liver,Lymph,Platelet,
- Western blot detection of ATP-Citrate Lyase in 3T3,K562,COS7 and Hela cell lysates using ATP-Citrate Lyase mouse mAb (1:1000 diluted).Predicted band size: 120KDa.Observed band size: 120KDa.
- Immunocytochemistry of HeLa cells using anti-ATP-Citrate Lyase (C-terminus) mouse mAb diluted 1:150.
- Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG
