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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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NFIC mouse mAb
- 货号:YM1253
- 应用:WB;ICC
- 种属:Human
- 免疫原:
- Purified recombinant human NFIC protein fragments expressed in E.coli.
- 特异性:
- This antibody detects endogenous levels of NFIC and does not cross-react with related proteins.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- 1110019L22Rik;1500041O16Rik;AA589446;AI746521;CAAT box transcription factor;CCAAT binding transcription factor;CCAAT box binding transcription factor;CCAAT-box-binding transcription factor;CNFI C;CTF;CTF5;MGC137374;MGC20153;NF I;NF I/C;NF-I/C;NF1 C;NF1-C;NF1C;NFI;NFI-C;NFI/C;NFIC;NFIC_HUMAN;Nuclear factor 1;Nuclear factor 1 C type;Nuclear factor 1 C-type;Nuclear factor 1/C;Nuclear factor I/C;TGGCA binding protein;TGGCA-binding protein;Transcription factor NFIC.
- 背景:
- The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011],
- 功能:
- function:Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.,similarity:Belongs to the CTF/NF-I family.,similarity:Contains 1 CTF/NF-I DNA-binding domain.,subunit:Binds DNA as a homodimer.,
- Western blot detection of NFIC in Hela NE cell lysates using NFIC mouse mAb (1:200 diluted).Predicted band size:56KDa.Observed band size:56KDa.
- Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-NFIC mouse mAb (dilution 1:200).