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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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DBC1 mouse mAb
- 货号:YM1212
- 应用:WB;ICC;IP
- 种属:Human;Mouse;Rat;Monkey
- 免疫原:
- Purified recombinant human DBC1 protein fragments expressed in E.coli
- 特异性:
- This antibody detects endogenous levels of DBC1 and does not cross-react with related proteins
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 纯化工艺:
- The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- DBCCR1;Deleted in bladder cancer protein 1;Deleted in bladder cancer protein 1 recursor;FAM5A;Protein FAM5A.
- 背景:
- tissue specificity:Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.,
- 功能:
- tissue specificity:Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.,
- 细胞定位:
- Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Recruited to chromatin, post-UV irradiation. Sequestered to the cytoplasm in the presence of MCC. Translocated to the cytoplasm during UV-induced apoptosis. .
- 组织表达:
- Expressed in gastric carcinoma tissue and the expression gradually increases with the progression of the carcinoma (at protein level). Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
- Western blot detection of DBC1 in HeLa,A549,Jurkat,C6,3T3 and COS7 cell lysates using DBC1 mouse mAb (1:500 diluted).Predicted band size:130KDa.Observed band size:130KDa.
- Immunocytochemistry of HeLa cells using anti-DBC1 mouse mAb diluted 1:200.
- Immunoprecipitation analysis of Hela cell lysates using DBC1 mouse mAb.