• 靶点：
• TFIIB

• 简介：
• >>Basal transcription factors;>>Spinocerebellar ataxia;>>Viral carcinogenesis

• 基因名称：
• GTF2B

• 蛋白名称：
• Transcription initiation factor IIB

• Human Gene Id：
• 2959

• Human Swiss Prot No：
• Q00403

• Mouse Gene Id：
• 229906

• Mouse Swiss Prot No：
• P62915

• Rat Gene Id：
• 81673

• Rat Swiss Prot No：
• P62916

• 免疫原：
• Purified recombinant human TFIIB protein fragments expressed in E.coli.

• 特异性：
• TFIIB Monoclonal Antibody detects endogenous levels of TFIIB protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:1000 - 1:2000. IHC 1:500 - 1:1000. IF 1:100 - 1:500. Not yet tested in other applications.

• 纯化工艺：
• Affinity purification

• 浓度：
• 1 mg/ml

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• GTF2B;TF2B;TFIIB;Transcription initiation factor IIB;General transcription factor TFIIB;S300-II

• 分子量：
• 35kD

• 背景：
• general transcription factor IIB(GTF2B) Homo sapiens This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008],

• 功能：
• cofactor:Binds 1 zinc ion per subunit.,function:General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.,similarity:Belongs to the TFIIB family.,similarity:Contains 1 TFIIB-type zinc finger.,subunit:Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 (By similarity). Interacts with Epstein-Barr virus EBNA2.,

• 细胞定位：
• Nucleus . Chromosome . Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171). .

• 组织表达：
• Expressed in the inner cell mass forming the embryoblast (PubMed:24441171). Not detected in cells from the outer thin layer trophoblast (at protein level) (PubMed:24441171).

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