PEPCK Monoclonal Antibody
- 货号：YM1074
- 应用：WB
- 种属：Human;Mouse;Rat;Bovine;Dog;Pig
- 简介：
- >>Glycolysis / Gluconeogenesis;>>Citrate cycle (TCA cycle);>>Pyruvate metabolism;>>Metabolic pathways;>>PPAR signaling pathway;>>FoxO signaling pathway;>>PI3K-Akt signaling pathway;>>AMPK signaling pathway;>>Insulin signaling pathway;>>Adipocytokine signaling pathway;>>Glucagon signaling pathway;>>Insulin resistance;>>Proximal tubule bicarbonate reclamation
- 蛋白名称：
- Phosphoenolpyruvate carboxykinase [GTP] mitochondrial
- 免疫原：
- Purified recombinant human PEPCK (C-terminus) protein fragments expressed in E.coli.
- 特异性：
- PEPCK Monoclonal Antibody detects endogenous levels of PEPCK protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:1000 - 1:2000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PCK2;PEPCK2;Phosphoenolpyruvate carboxykinase [GTP]; mitochondrial;PEPCK-M;Phosphoenolpyruvate carboxylase
- 背景：
- This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014],
- 功能：
- catalytic activity:GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO(2).,cofactor:Binds 1 manganese ion per subunit.,cofactor:Manganese.,disease:Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (mitochondrial PEPCK deficiency) [MIM:261650]. PEPCK deficiency is a metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycaemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.,function:Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid
- 组织表达：
- Liver,Neuroblastoma,Placenta,
- Western Blot analysis using PEPCK Monoclonal Antibody against ZR75-1, HepG2, HeLa cell lysate.
