PA28γ Monoclonal Antibody
- 货号：YM1070
- 应用：WB;IF
- 种属：Human;Mouse;Dog;Pig
- 简介：
- >>Proteasome;>>Antigen processing and presentation;>>Hepatitis C
- 蛋白名称：
- Proteasome activator complex subunit 3
- 免疫原：
- Purified recombinant human PA28γ protein fragments expressed in E.coli.
- 特异性：
- PA28γ Monoclonal Antibody detects endogenous levels of PA28γ protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:1000 - 1:2000. IF 1:100 - 1:500. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PSME3;Proteasome activator complex subunit 3;11S regulator complex subunit gamma;REG-gamma;Activator of multicatalytic protease subunit 3;Ki nuclear autoantigen;Proteasome activator 28 subunit gamma;PA28g;PA28gamma
- 背景：
- The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) o
- 功能：
- disease:Sera from patients with systemic lupus erythematosus often contain antibodies that react with the Ki antigen.,domain:The C-terminal sequences affect heptamer stability and proteasome affinity.,function:Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a donut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-TP53/p53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53/p53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation.,induction:Up-regulated in thyroid carcinoma cells.,PTM:Phosphorylated by MAP3K3.,similarity:Belongs to the PA28 family.,subcellular lo
- 细胞定位：
- Nucleus . Cytoplasm . Localizes to the cytoplasm during mitosis following nuclear envelope breakdown at this distinct stage of the cell cycle which allows its interaction with MAP3K3 kinase. .
- 组织表达：
- B-cell,Embryonic kidney,Fetal brain,Human endometrium carcinoma cell line,L
- Western Blot analysis using PA28γ Monoclonal Antibody against HepG2, Jurkat, K562 cell lysate.
