TRIM5α Monoclonal Antibody
- 货号：YM0627
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Viral life cycle - HIV-1;>>Human immunodeficiency virus 1 infection
- 蛋白名称：
- Tripartite motif-containing protein 5
- 免疫原：
- Purified recombinant fragment of human TRIM5α expressed in E. Coli.
- 特异性：
- TRIM5α Monoclonal Antibody detects endogenous levels of TRIM5α protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- TRIM5;RNF88;Tripartite motif-containing protein 5;RING finger protein 88
- 背景：
- The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2009],
- 功能：
- domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.,function:Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.,pathway:Protein modification; protein ubiquitination.,PTM:Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).,similarity:Belongs to the TRIM/RBCC family.,similarity:Contains 1 B box-type zinc finger.,similarity:Contains 1 B30.2/SPRY domain.,similarity
- 细胞定位：
- Cytoplasm . Nucleus . Predominantly localizes in cytoplasmic bodies (PubMed:12878161, PubMed:20357094). Localization may be influenced by the coexpression of other TRIM proteins, hence partial nuclear localization is observed in the presence of TRIM22 or TRIM27 (By similarity). In cytoplasmic bodies, colocalizes with proteasomal subunits and SQSTM1 (By similarity). .
- 组织表达：
- Brain,Placenta,Rhabdomyosarcoma,
- Western Blot analysis using TRIM5α Monoclonal Antibody against human breast carcinoma tissue lysate.
- Immunohistochemistry analysis of paraffin-embedded human metastatic adenocarcinoma(A) and stomach adenocarcinoma (B), showing cytoplasmic localization with AEC staining (A) and DAB staining(B) using TRIM5α Monoclonal Antibody.
