S-100 α Monoclonal Antibody
- 货号：YM0571
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 免疫原：
- Purified recombinant fragment of S-100 α expressed in E. Coli.
- 特异性：
- S-100 α Monoclonal Antibody detects endogenous levels of S-100 α protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- S100A1;S100A;Protein S100-A1;S-100 protein alpha chain;S-100 protein subunit alpha;S100 calcium-binding protein A1
- 背景：
- S100 calcium binding protein A1(S100A1) Homo sapiens The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-induced Ca2+ release, inhibition of microtubule assembly, and inhibition of protein kinase C-mediated phosphorylation. Reduced expression of this protein has been implicated in cardiomyopathies. [provided by RefSeq, Jul 2008],
- 功能：
- function:Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.,similarity:Belongs to the S-100 family.,similarity:Contains 2 EF-hand domains.,subunit:Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain.,tissue specificity:Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.,
- 细胞定位：
- Cytoplasm . Sarcoplasmic reticulum . Mitochondrion .
- 组织表达：
- Highly prevalent in heart (PubMed:12804600, PubMed:1384693). Also found in lesser quantities in skeletal muscle and brain (PubMed:1384693).
- Western Blot analysis using S-100 α Monoclonal Antibody against truncated S-100 α recombinant protein.
- Immunohistochemistry analysis of paraffin-embedded human brain tissue (A), lymphoid follicles tissue (B) and interbrain tissue (C), showing cytoplasmic localization with DAB staining using S-100 α Monoclonal Antibody.
