Rsk-2 Monoclonal Antibody
- 货号：YM0565
- 应用：WB;IF;FCM;ELISA
- 种属：Human
- 简介：
- >>MAPK signaling pathway;>>Oocyte meiosis;>>mTOR signaling pathway;>>Thermogenesis;>>Long-term potentiation;>>Neurotrophin signaling pathway;>>Progesterone-mediated oocyte maturation;>>Insulin resistance;>>Yersinia infection;>>Chemical carcinogenesis - receptor activation
- 蛋白名称：
- Ribosomal protein S6 kinase alpha-3
- 免疫原：
- Purified recombinant fragment of human Rsk-2 expressed in E. Coli.
- 特异性：
- Rsk-2 Monoclonal Antibody detects endogenous levels of Rsk-2 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- RPS6KA3;ISPK1;MAPKAPK1B;RSK2;Ribosomal protein S6 kinase alpha-3;S6K-alpha-3;90 kDa ribosomal protein S6 kinase 3;p90-RSK 3;p90RSK3;Insulin-stimulated protein kinase 1;ISPK-1;MAP kinase-activated protein kinase 1b;MAPK-activated
- 背景：
- ribosomal protein S6 kinase A3(RPS6KA3) Homo sapiens This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq, Jul 2008],
- 功能：
- catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,disease:Defects in RPS6KA3 are the cause of Coffin-Lowry syndrome (CLS) [MIM:303600]; an X-linked dominant disorder characterized by severe mental retardation with facial and digital dysmorphisms, and progressive skeletal deformations.,enzyme regulation:Activated by multiple phosphorylations on threonine and serine residues.,function:Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.,PTM:Autophosphorylated on Ser-386, as part of the activation process.,PTM:Ser-227 phosphorylation promotes Ser-386 phosphorylation and leads to basal activation. Full activation by growth factors requires additional phosphorylation on Ser-369.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase
- 细胞定位：
- Nucleus . Cytoplasm .
- 组织表达：
- Expressed in many tissues, highest levels in skeletal muscle.
- Western Blot analysis using Rsk-2 Monoclonal Antibody against HeLa (1), MCF-7 (2), and HepG2 (3) cell lysate.
- Immunofluorescence analysis of HepG2 cells using Rsk-2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of HepG2 cells using Rsk-2 Monoclonal Antibody (green) and negative control (red).
