ROR1 Monoclonal Antibody

    • 货号:YM0563
    • 应用:WB;IF;ELISA
    • 种属:Human
      • 靶点:
      • ROR1
      • 简介:
      • >>Wnt signaling pathway
      • 基因名称:
      • ROR1
      • 蛋白名称:
      • Tyrosine-protein kinase transmembrane receptor ROR1
      • Human Gene Id:
      • 4919
      • Human Swiss Prot No:
      • Q01973
      • Mouse Swiss Prot No:
      • Q9Z139
      • 免疫原:
      • Recombinant extracellular fragment of human ROR1 (aa30-406) fused with hIgGFc tag, expressed in HEK293 cells
      • 特异性:
      • ROR1 Monoclonal Antibody detects endogenous levels of ROR1 protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • Affinity purification
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • ROR1;NTRKR1;Tyrosine-protein kinase transmembrane receptor ROR1;Neurotrophic tyrosine kinase; receptor-related 1
      • 分子量:
      • 104kD
      • 背景:
      • This gene encodes a receptor tyrosine kinase-like orphan receptor that modulates neurite growth in the central nervous system. The encoded protein is a glycosylated type I membrane protein that belongs to the ROR subfamily of cell surface receptors. It is a pseudokinase that lacks catalytic activity and may interact with the non-canonical Wnt signalling pathway. This gene is highly expressed during early embryonic development but expressed at very low levels in adult tissues. Increased expression of this gene is associated with B-cell chronic lymphocytic leukaemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2012],
      • 功能:
      • catalytic activity:ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.,developmental stage:Expressed at high levels during early embryonic development. The expression levels drop strongly around day 16 and there are only very low levels in adult tissues.,function:Tyrosine-protein kinase receptor whose role is not yet clear.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. ROR subfamily.,similarity:Contains 1 FZ (frizzled) domain.,similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain.,similarity:Contains 1 kringle domain.,similarity:Contains 1 protein kinase domain.,tissue specificity:Expressed strongly in human heart, lung, and kidney, but weakly in the CNS. The short isoform is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.,
      • 细胞定位:
      • Membrane ; Single-pass type I membrane protein. Cell projection, axon .
      • 组织表达:
      • Expressed strongly in human heart, lung and kidney, but weakly in the CNS. Isoform Short is strongly expressed in fetal and adult CNS and in a variety of human cancers, including those originating from CNS or PNS neuroectoderm.
      • 产品图片
      • Western Blot analysis using ROR1 Monoclonal Antibody against extracellular domain of human ROR1 (aa30-423).
      • Confocal immunofluorescence analysis of HEK293 cells trasfected with extracellular ROR1 (aa30-406)-hIgGFc using ROR1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.