• 靶点：
• PRK2

• 简介：
• >>PI3K-Akt signaling pathway;>>NOD-like receptor signaling pathway;>>Yersinia infection

• 基因名称：
• PKN2

• 蛋白名称：
• Serine/threonine-protein kinase N2

• Human Gene Id：
• 5586

• Human Swiss Prot No：
• Q16513

• Mouse Gene Id：
• 109333

• Mouse Swiss Prot No：
• Q8BWW9

• Rat Swiss Prot No：
• O08874

• 免疫原：
• Purified recombinant fragment of human PRK2 expressed in E. Coli.

• 特异性：
• PRK2 Monoclonal Antibody detects endogenous levels of PRK2 protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:500 - 1:2000. IHC 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000.. IF 1:50-200

• 纯化工艺：
• Affinity purification

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• PKN2;PRK2;PRKCL2;Serine/threonine-protein kinase N2;PKN gamma;Protein kinase C-like 2;Protein-kinase C-related kinase 2

• 分子量：
• 112kD

• 背景：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites, Thr-816 (activation loop of the kinase domain) and Thr-958 (turn motif), need to be phosphorylated for its full activation.,function:Exhibits a preference for highly basic protein substrates.,PTM:Activated by limited proteolysis with trypsin.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 REM (Hr1) repeats.,

• 功能：
• catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C1 domain does not bind the diacylglycerol (DAG).,enzyme regulation:Activated by lipids, particularly cardiolipin and to a lesser extent by other acidic phospholipids and unsaturated fatty acids. Two specific sites, Thr-816 (activation loop of the kinase domain) and Thr-958 (turn motif), need to be phosphorylated for its full activation.,function:Exhibits a preference for highly basic protein substrates.,PTM:Activated by limited proteolysis with trypsin.,PTM:Autophosphorylated.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 C2 domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 3 REM (Hr1) repeats.,

• 细胞定位：
• Cytoplasm . Nucleus . Membrane . Cell projection, lamellipodium . Cytoplasm, cytoskeleton . Cleavage furrow . Midbody . Cell junction . Colocalizes with PTPN13 in lamellipodia-like structures, regions of large actin turnover. Accumulates during telophase at the cleavage furrow and concentrates finally around the midbody in cytokinesis. Recruited to nascent cell-cell contacts at the apical surface of cells. In the course of viral infection, colocalizes with HCV NS5B at perinuclear region in the cytoplasm. .

• 组织表达：
• Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.

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