NGFR p75 Monoclonal Antibody
- 货号：YM0475
- 应用：WB;IF;FCM;ELISA
- 种属：Human
- 简介：
- >>MAPK signaling pathway;>>Ras signaling pathway;>>Rap1 signaling pathway;>>Cytokine-cytokine receptor interaction;>>PI3K-Akt signaling pathway;>>Apoptosis - multiple species;>>Neurotrophin signaling pathway;>>Transcriptional misregulation in cancer
- 蛋白名称：
- Tumor necrosis factor receptor superfamily member 16
- 免疫原：
- Purified recombinant fragment of human NGFR p75 expressed in E. Coli.
- 特异性：
- NGFR p75 Monoclonal Antibody detects endogenous levels of NGFR p75 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NGFR;TNFRSF16;Tumor necrosis factor receptor superfamily member 16;Gp80-LNGFR;Low affinity neurotrophin receptor p75NTR;Low-affinity nerve growth factor receptor;NGF receptor;p75 ICD;CD antigen CD271
- 背景：
- Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008],
- 功能：
- domain:Death domain is responsible for interaction with RANBP9.,domain:The extracellular domain is responsible for interaction with NTRK1.,function:Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.,PTM:N- and O-glycosylated.,PTM:O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.,PTM:Phosphorylated on serine residues.,similarity:Contains 1 death domain.,similarity:Contains 4 TNFR-Cys repeats.,subunit:Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3. Interacts with KIDINS220 and NTRK1. Can form a ternary complex with NTRK1 and KIDINS220 and this complex is affected by the expression levels of KIDI
- 细胞定位：
- Cell membrane ; Single-pass type I membrane protein . Perikaryon . Cell projection, growth cone . Cell projection, dendritic spine .
- Western Blot analysis using NGFR p75 Monoclonal Antibody against NGFR-hIgGFc transfected HEK293 cell lysate.
- Immunofluorescence analysis of EC cells using NGFR p75 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
- Flow cytometric analysis of EC cells using NGFR p75 Monoclonal Antibody (green) and negative control (purple).
