N-CoR Monoclonal Antibody
- 货号：YM0467
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Endocrine resistance;>>Thyroid hormone signaling pathway;>>Transcriptional misregulation in cancer
- 蛋白名称：
- Nuclear receptor corepressor 1
- 免疫原：
- Purified recombinant fragment of N-CoR (aa1-192) expressed in E. Coli.
- 特异性：
- N-CoR Monoclonal Antibody detects endogenous levels of N-CoR protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- NCOR1;KIAA1047;Nuclear receptor corepressor 1;N-CoR;N-CoR1
- 背景：
- This gene encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. This gene is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 17 and 20.[provided by RefSeq, Jun 2010],
- 功能：
- domain:The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.,domain:The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).,function:Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.,PTM:Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.,similarity:Belongs to the N-CoR nuclear receptor corepres
- 组织表达：
- Brain,Colon,Epithelium,Fetal brain,Lung,Ovary,Pancreas,Pooled,Skin,Testis,
- Western Blot analysis using N-CoR Monoclonal Antibody against truncated Trx-N-CoR recombinant protein (1).
- Immunohistochemistry analysis of paraffin-embedded human cerebra (left) and breast carcinoma tissue (right), showing nuclear location with DAB staining using N-CoR Monoclonal Antibody.
