Mi2-α Monoclonal Antibody
- 货号：YM0442
- 应用：WB;IHC;IF;FCM;ELISA
- 种属：Human;Mouse
- 蛋白名称：
- Chromodomain-helicase-DNA-binding protein 3
- 免疫原：
- Purified recombinant fragment of human Mi2-α expressed in E. Coli.
- 特异性：
- Mi2-α Monoclonal Antibody detects endogenous levels of Mi2-α protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CHD3;Chromodomain-helicase-DNA-binding protein 3;CHD-3;ATP-dependent helicase CHD3;Mi-2 autoantigen 240 kDa protein;Mi2-alpha;Zinc finger helicase;hZFH
- 背景：
- This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
- 功能：
- disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,function:Probable transcription regulator.,sequence caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed.,
- 细胞定位：
- Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. .
- Western Blot analysis using Mi2-α Monoclonal Antibody against HeLa (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
- Immunohistochemistry analysis of paraffin-embedded colon cancer tissues with DAB staining using Mi2-α Monoclonal Antibody.
- Immunofluorescence analysis of Hela cells using Mi2-α Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
- Flow cytometric analysis of K562 cells using Mi2-α Monoclonal Antibody (green) and negative control (purple).
