Mi2-α Monoclonal Antibody

    • 货号:YM0442
    • 应用:WB;IHC;IF;FCM;ELISA
    • 种属:Human;Mouse
      • 靶点:
      • Mi2-α
      • 基因名称:
      • CHD3
      • 蛋白名称:
      • Chromodomain-helicase-DNA-binding protein 3
      • Human Gene Id:
      • 1107
      • Human Swiss Prot No:
      • Q12873
      • 免疫原:
      • Purified recombinant fragment of human Mi2-α expressed in E. Coli.
      • 特异性:
      • Mi2-α Monoclonal Antibody detects endogenous levels of Mi2-α protein.
      • 组成:
      • Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
      • 来源:
      • Monoclonal, Mouse
      • 稀释:
      • WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
      • 纯化工艺:
      • Affinity purification
      • 储存:
      • -15°C to -25°C/1 year(Do not lower than -25°C)
      • 其他名称:
      • CHD3;Chromodomain-helicase-DNA-binding protein 3;CHD-3;ATP-dependent helicase CHD3;Mi-2 autoantigen 240 kDa protein;Mi2-alpha;Zinc finger helicase;hZFH
      • 分子量:
      • 227kD
      • 背景:
      • This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008],
      • 功能:
      • disease:One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.,function:Probable transcription regulator.,sequence caution:Differs from position 1967 onward for unknown reasons.,similarity:Belongs to the SNF2/RAD54 helicase family.,similarity:Contains 1 helicase ATP-binding domain.,similarity:Contains 1 helicase C-terminal domain.,similarity:Contains 2 chromo domains.,similarity:Contains 2 PHD-type zinc fingers.,subunit:Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts via its C-terminal region with HABP4.,tissue specificity:Widely expressed.,
      • 细胞定位:
      • Nucleus, PML body . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with centrosomes in interphase and mitosis. .
      • 组织表达:
      • Widely expressed.
      • 产品图片
      • Western Blot analysis using Mi2-α Monoclonal Antibody against HeLa (1), K562 (2), Jurkat (3), NTERA-2 (4), HEK293 (5), Raji (6) cell lysate and mouse brain (7) tissue lysate.
      • Immunohistochemistry analysis of paraffin-embedded colon cancer tissues with DAB staining using Mi2-α Monoclonal Antibody.
      • Immunofluorescence analysis of Hela cells using Mi2-α Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
      • Flow cytometric analysis of K562 cells using Mi2-α Monoclonal Antibody (green) and negative control (purple).