LysRS Monoclonal Antibody
- 货号:YM0425
- 应用:WB;IHC;IF;ELISA
- 种属:Human
- 简介:
- >>Aminoacyl-tRNA biosynthesis
- 蛋白名称:
- Lysyl-tRNA synthetase
- 免疫原:
- Purified recombinant fragment of LysRS(aa90-174) expressed in E. Coli.
- 特异性:
- LysRS Monoclonal Antibody detects endogenous levels of LysRS protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- KARS;KIAA0070;Lysine--tRNA ligase;Lysyl-tRNA synthetase;LysRS
- 背景:
- Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase is a homodimer localized to the cytoplasm which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys).,similarity:Belongs to the class-II aminoacyl-tRNA synthetase family.,subunit:Homodimer (By similarity); also part of a multisubunit complex that groups tRNA ligases for Arg, Asp, Glu, Gln, Ile, Leu, Lys, Met and Pro.,
- 细胞定位:
- [Isoform Cytoplasmic]: Cytoplasm, cytosol . Cytoplasm . Nucleus . Cell membrane ; Peripheral membrane protein . Secreted . Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739). .; [Isoform Mitochondrial]: Mitochondrion .
- 组织表达:
- Bone marrow,Brain,Cerebellum,Hepatoma,Placenta,
![](/uploadfiles/PIC/ym0425-wb.jpg)
- Western Blot analysis using LysRS Monoclonal Antibody against truncated Trx-LysRS recombinant protein (1), truncated MBP-LysRS (aa90-174) and full length LysRS (aa1-188) transfected CHO-K1 cell lysate (3).
![](/uploadfiles/PIC/ym0425-ihc.jpg)
- Immunohistochemistry analysis of paraffin-embedded human cervical carcinoma, showing cytoplasmic localization with DAB staining using LysRS Monoclonal Antibody.