KLHL22 Monoclonal Antibody
- 货号：YM0403
- 应用：WB;IF;ELISA
- 种属：Human
- 蛋白名称：
- Kelch-like protein 22
- 免疫原：
- Purified recombinant fragment of human KLHL22 expressed in E. Coli.
- 特异性：
- KLHL22 Monoclonal Antibody detects endogenous levels of KLHL22 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KLHL22;Kelch-like protein 22
- 背景：
- similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,
- 功能：
- similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,
- 细胞定位：
- Cytoplasm, cytosol . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Nucleus . Lysosome . Mainly cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (PubMed:23455478). Localizes to the nucleus upon amino acid starvation (PubMed:29769719). Relocalizes to the cytosol and associates with lysosomes when amino acids are available (PubMed:29769719). .
- 组织表达：
- Embryo,Kidney,Liver,
- Western Blot analysis using KLHL22 Monoclonal Antibody against mouse brain tissues lysate.
- Immunofluorescence analysis of U251 cells using KLHL22 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
