KLHL13 Monoclonal Antibody
- 货号：YM0401
- 应用：WB;IHC;IF;FCM;ELISA
- 种属：Human
- 简介：
- >>Ubiquitin mediated proteolysis
- 蛋白名称：
- Kelch-like protein 13
- 免疫原：
- Purified recombinant fragment of human KLHL13 expressed in E. Coli.
- 特异性：
- KLHL13 Monoclonal Antibody detects endogenous levels of KLHL13 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KLHL13;BKLHD2;KIAA1309;Kelch-like protein 13;BTB and kelch domain-containing protein 2
- 背景：
- This gene encodes a BTB and kelch domain containing protein and belongs to the kelch repeat domain containing superfamily of proteins. The encoded protein functions as an adaptor protein that complexes with Cullin 3 and other proteins to form the Cullin 3-based E3 ubiquitin-protein ligase complex. This complex is necessary for proper chromosome segregation and completion of cytokinesis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010],
- 功能：
- function:Substrate-specific adapter for a CUL3-based E3 ubiquitin-protein ligase complex. Within this complex, controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.,pathway:Protein modification; protein ubiquitination.,similarity:Contains 1 BACK (BTB/Kelch associated) domain.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subunit:Forms a complex with CUL3 and KLHL9. Interacts with AURKB. Interacts with CUL3.,
- 细胞定位：
- midbody,Cul3-RING ubiquitin ligase complex,
- 组织表达：
- Brain,Testis,Thalamus,Whole embryo,
- Western Blot analysis using KLHL13 Monoclonal Antibody against HeLa (1) and MCF-7 (2) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded brain tissues (left) and pancreas tissues (right) with DAB staining using KLHL13 Monoclonal Antibody.
- Immunofluorescence analysis of NTERA-2 cells (left) and U251 (right) cells using KLHL13 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of 3T3/L1 cells using KLHL13 Monoclonal Antibody (green) and negative control (purple).
