KLHL12 Monoclonal Antibody
- 货号：YM0400
- 应用：WB;ELISA
- 种属：Human
- 蛋白名称：
- Kelch-like protein 12
- 免疫原：
- Purified recombinant fragment of human KLHL12 expressed in E. Coli.
- 特异性：
- KLHL12 Monoclonal Antibody detects endogenous levels of KLHL12 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KLHL12;C3IP1;Kelch-like protein 12;CUL3-interacting protein 1;DKIR homolog;hDKIR
- 背景：
- This gene encodes a member of the KLHL (Kelch-like) family of proteins. This protein has been identified as an autoantigen in the autoimmune disease Sjogren's syndrome and as a potential biomarker in primary biliary cirrhosis. This protein may act as a substrate adaptor of the Cullin-3 ubiquitin ligase complex to promote substrate-specific ubiquitylation. Ubiquitylation by this complex has been shown to regulate the Wnt signaling pathway as well as COPII vesicle coat size. A pseudogene has been identified on chromosome 22. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014],
- 功能：
- domain:The BTB domain is required for interaction with CUL3.,function:Serves as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex. Negatively regulates the Wnt signaling pathway via the targeted ubiquitination and subsequent proteolysis of DVL3.,similarity:Contains 1 BTB (POZ) domain.,similarity:Contains 6 Kelch repeats.,subunit:Component of an ubiquitin-protein E3 ligase complex which includes at least CUL3 and KLHL12. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A.,tissue specificity:Highly expressed in testis and at lower levels in the submandibular salivary gland.,
- 细胞定位：
- Cytoplasmic vesicle, COPII-coated vesicle .
- 组织表达：
- Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland.
- Western Blot analysis using KLHL12 Monoclonal Antibody against HeLa (1) cell lysate.
