JMJD2A Monoclonal Antibody
- 货号：YM0389
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 蛋白名称：
- Lysine-specific demethylase 4A
- 免疫原：
- Purified recombinant fragment of human JMJD2A expressed in E. Coli.
- 特异性：
- JMJD2A Monoclonal Antibody detects endogenous levels of JMJD2A protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KDM4A;JHDM3A;JMJD2;JMJD2A;KIAA0677;Lysine-specific demethylase 4A;JmjC domain-containing histone demethylation protein 3A;Jumonji domain-containing protein 2A
- 背景：
- This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. [provided by RefSeq, Apr 2009],
- 功能：
- cofactor:Binds 1 Fe(2+) ion per subunit.,domain:The 2 Tudor domains recognize and bind methylated histone H3 'Lys-4' residue. Double Tudor domain has an interdigitated structure and the unusual fold is required for its ability to bind methylated histone tails. Trimethylated H3 'Lys-4' is bound in a cage of 3 aromatic residues, 2 of which are from the Tudor domain 2, while the binding specificity is determined by side-chain interactions involving residues from the Tudor domain 1. The Tudor domains are able to bind trimethylated histone H3 'Lys-4', trimethylated histone H3 'Lys-9', di- and trimethylated H4 'Lys-20'.,function:Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' r
- Western Blot analysis using JMJD2A Monoclonal Antibody against HEK293 (1) and JMJD2A-hIgGFc transfected HEK293 (2) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded colon cancer tissues (left) and human larynx cancer tissues (right) with DAB staining using JMJD2A Monoclonal Antibody.
- Immunofluorescence analysis of NTERA-2 cells using JMJD2A Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
