Islet-1 Monoclonal Antibody
- 货号：YM0382
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 简介：
- >>Signaling pathways regulating pluripotency of stem cells
- 蛋白名称：
- Insulin gene enhancer protein ISL-1
- 免疫原：
- Purified recombinant fragment of human Islet-1 expressed in E. Coli.
- 特异性：
- Islet-1 Monoclonal Antibody detects endogenous levels of Islet-1 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- ISL1;Insulin gene enhancer protein ISL-1;Islet-1
- 背景：
- This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008],
- 功能：
- function:Binds to one of the cis-acting domain of the insulin gene enhancer.,similarity:Contains 1 homeobox DNA-binding domain.,similarity:Contains 2 LIM zinc-binding domains.,tissue specificity:Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.,
- 组织表达：
- Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
- Western Blot analysis using Islet-1 Monoclonal Antibody against full-length ISL1 (aa1-349)-hIgGFc transfected HEK293 cell lysate(1).
- Immunohistochemistry analysis of paraffin-embedded human lung cancer (left) and cervical carcinoma (right), showing nuclear localization with DAB staining using Islet-1 Monoclonal Antibody.
- Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length ISL1-hIgGFc using Islet-1 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
