IL-34 Monoclonal Antibody
- 货号:YM0371
- 应用:WB;IF;FCM;ELISA
- 种属:Human
- 简介:
- >>Cytokine-cytokine receptor interaction;>>Viral protein interaction with cytokine and cytokine receptor
- 免疫原:
- Purified recombinant fragment of human IL-34 expressed in E. Coli.
- 特异性:
- IL-34 Monoclonal Antibody detects endogenous levels of IL-34 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- IL34;C16orf77;Interleukin-34;IL-34
- 背景:
- Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008],
- 功能:
- function:Cytokine that promotes the differentiation and viability of monocytes and macrophages. Stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Ligand for colony-stimulating factor-1 receptor CSF1R.,similarity:Belongs to the IL-34 family.,subunit:Homodimer.,tissue specificity:Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.,
- 组织表达:
- Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.
- Western Blot analysis using IL-34 Monoclonal Antibody against IL-34-hIgGFc transfected HEK293 cell lysate.
- Immunofluorescence analysis of U251 cells using IL-34 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of K562 cells using IL-34 Monoclonal Antibody (green) and negative control (purple).