• 靶点：
• HPRT

• 简介：
• >>Purine metabolism;>>Drug metabolism - other enzymes;>>Metabolic pathways;>>Nucleotide metabolism

• 基因名称：
• HPRT1

• 蛋白名称：
• Hypoxanthine-guanine phosphoribosyltransferase

• Human Gene Id：
• 3251

• Human Swiss Prot No：
• P00492

• Mouse Swiss Prot No：
• P00493

• 免疫原：
• Purified recombinant fragment of HPRT expressed in E. Coli.

• 特异性：
• HPRT Monoclonal Antibody detects endogenous levels of HPRT protein.

• 组成：
• Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

• 来源：
• Monoclonal, Mouse

• 稀释：
• WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

• 纯化工艺：
• Affinity purification

• 储存：
• -15°C to -25°C/1 year(Do not lower than -25°C)

• 其他名称：
• HPRT1;HPRT;Hypoxanthine-guanine phosphoribosyltransferase;HGPRT;HGPRTase

• 分子量：
• 25kD

• 背景：
• hypoxanthine phosphoribosyltransferase 1(HPRT1) Homo sapiens The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.[provided by RefSeq, Jun 2009],

• 功能：
• catalytic activity:GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.,catalytic activity:IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.,cofactor:Binds 2 magnesium ions per subunit. One of the ions does not make direct protein contacts.,disease:Defects in HPRT1 are the cause of gout [MIM:300323]; also known as HPRT-related gout or Kelley-Seegmiller syndrome. Gout is characterized by partial enzyme activity and hyperuricemia.,disease:Defects in HPRT1 are the cause of Lesch-Nyhan syndrome (LNS) [MIM:300322]. LNS is characterized by complete lack of enzymatic activity that results in hyperuricemia, choreoathetosis, mental retardation, and compulsive self-mutilation.,online information:Hypoxanthine-guanine phosphoribosyltransferase entry,pathway:Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.,similarity:B

• 细胞定位：
• Cytoplasm.

• 组织表达：
• Brain,

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