GRK 2 Monoclonal Antibody
- 货号:YM0314
- 应用:WB;IHC;IF;ELISA
- 种属:Human;Mouse;Rat;Monkey
- 简介:
- >>Chemokine signaling pathway;>>Endocytosis;>>Hedgehog signaling pathway;>>Glutamatergic synapse;>>Olfactory transduction;>>Morphine addiction
- 蛋白名称:
- Beta-adrenergic receptor kinase 1
- 免疫原:
- Purified recombinant fragment of human GRK 2 expressed in E. Coli.
- 特异性:
- GRK 2 Monoclonal Antibody detects endogenous levels of GRK 2 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- ADRBK1;BARK;BARK1;GRK2;Beta-adrenergic receptor kinase 1;Beta-ARK-1;G-protein coupled receptor kinase 2
- 背景:
- The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],
- 功能:
- catalytic activity:ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate.,catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them.,online information:Beta adrenergic receptor kinase entry,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 RGS domain.,subunit:Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5.,tissue specificity:Expressed in peripheral blood leukocytes.,
- 细胞定位:
- Cytoplasm . Cell membrane . Cell junction, synapse, postsynapse . Cell junction, synapse, presynapse .
- 组织表达:
- Expressed in peripheral blood leukocytes.
![](/uploadfiles/PIC/ym0314-wb.jpg)
- Western Blot analysis using GRK 2 Monoclonal Antibody against HeLa (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.
![](/uploadfiles/PIC/ym0314-ihc.jpg)
- Immunohistochemistry analysis of paraffin-embedded endometrial cancer tissues (left) and cervical cancer tissues (right) with DAB staining using GRK 2 Monoclonal Antibody.
![](/uploadfiles/PIC/ym0314-if.jpg)
- Immunofluorescence analysis of NIH/3T3 cells using GRK 2 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.