Cytokeratin (Pan) Monoclonal Antibody
- 货号：YM0191
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 蛋白名称：
- Keratin type II cytoskeletal 5
- 免疫原：
- Purified recombinant fragment of Cytokeratin 5 expressed in E. Coli.
- 特异性：
- Cytokeratin (Pan) Monoclonal Antibody detects endogenous levels of Cytokeratin (Pan) protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- KRT5;Keratin; type II cytoskeletal 5;58 kDa cytokeratin;Cytokeratin-5;CK-5;Keratin-5;K5;Type-II keratin Kb5
- 背景：
- keratin 5(KRT5) Homo sapiens The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008],
- 功能：
- disease:Defects in KRT5 are a cause of epidermolysis bullosa simplex Dowling-Meara type (DM-EBS) [MIM:131760]. DM-EBS is a severe form of intraepidermal epidermolysis bullosa characterized by generalized herpetiform blistering, milia formation, dystrophic nails, and mucous membrane involvement.,disease:Defects in KRT5 are a cause of epidermolysis bullosa simplex Koebner type (K-EBS) [MIM:131900]. K-EBS is a form of intraepidermal epidermolysis bullosa characterized by generalized skin blistering. The phenotype is not fundamentally distinct from the Dowling-Meara type, althought it is less severe.,disease:Defects in KRT5 are a cause of epidermolysis bullosa simplex Weber-Cockayne type (WC-EBS) [MIM:131800]. WC-EBS is a form of intraepidermal epidermolysis bullosa characterized by blistering limited to palmar and plantar areas of the skin.,disease:Defects in KRT5 are the cause of Dowling-D
- 细胞定位：
- nucleus,cytoplasm,mitochondrion,cytosol,intermediate filament,plasma membrane,membrane,keratin filament,extracellular exosome,
- 组织表达：
- Expressed in corneal epithelium (at protein level).
- Western Blot analysis using Cytokeratin (Pan) Monoclonal Antibody against truncated CK5 recombinant protein.
- Immunohistochemistry analysis of paraffin-embedded human lung squamous cell carcinoma (A), normal hepatocyte (B), colon adenocacinoma (C), normal stomach tissue (D), showing cytoplasmic and membrane localization with DAB staining using Cytokeratin (Pan) M
- Immunofluorescence staining of methanol-fixed Eca-109 (left) and HepG2 (right) cells showing cytoplasmic localization using Cytokeratin (Pan) Monoclonal Antibody.
