CD71/TfR Monoclonal Antibody
- 货号：YM0132
- 应用：WB;IHC;IF;FCM;ELISA
- 种属：Human
- 简介：
- >>HIF-1 signaling pathway;>>Endocytosis;>>Phagosome;>>Ferroptosis;>>Hematopoietic cell lineage
- 蛋白名称：
- Transferrin receptor protein 1
- 免疫原：
- Purified recombinant fragment of human CD71 expressed in E. Coli.
- 特异性：
- CD71 Monoclonal Antibody detects endogenous levels of CD71 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- TFRC;Transferrin receptor protein 1;TR;TfR;TfR1;Trfr;T9;p90;CD antigen CD71
- 背景：
- This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015],
- 功能：
- function:Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.,induction:Regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation.,miscellaneous:Canine and feline parvoviruses bind human and feline transferrin receptors and use t
- 细胞定位：
- Cell membrane ; Single-pass type II membrane protein . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV. .; [Transferrin receptor protein 1, serum form]: Secreted .
- 组织表达：
- Brain,Epithelium,Erythroleukemia,Eye,Human endometrium carcinoma cell line,Liver,Pl
- Western Blot analysis using CD71 Monoclonal Antibody against HEK293 (1) and MBP-hIgGFc transfected HEK293 (2) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded brain tissues with DAB staining using CD71 Monoclonal Antibody.
- Immunofluorescence analysis of MSCS cells using CD71 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Flow cytometric analysis of HepG2 cells using CD71 Monoclonal Antibody (green) and negative control (red).
