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WB 试剂
- PonceauS Staining Solution
- PBST Washing Buffer, 10X
- 1.5M Tris-HCl Buffer, pH8.8
- 1M Tris-HCl Buffer, pH6.8
- 10% SDS Solution
- Prestained Protein Marker
- TBST Washing Buffer, 10X
- SDS PAGE Loading Buffer, 5X
- Stripping Buffered Solution
- Tris Buffer, pH7.4, 10X
- Total Protein Extraction Kit
- Running Buffer, 10X
- Transfer Buffer, 10X
- 30% Acr-Bis(29:1) Solution
- Tris电泳液速溶颗粒
- PBS(1X, premixed powder)
- TBS(1X, premixed powder)
- 快速封闭液
- 转膜液速溶颗粒
- Chemical reagents
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CD69 Monoclonal Antibody
- 货号:YM0131
- 应用:WB;IHC;IF;FCM;ELISA
- 种属:Human
- 蛋白名称:
- Early activation antigen CD69
- 免疫原:
- Purified recombinant fragment of human CD69 expressed in E. Coli.
- 特异性:
- CD69 Monoclonal Antibody detects endogenous levels of CD69 protein.
- 组成:
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释:
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺:
- Affinity purification
- 储存:
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称:
- CD69;CLEC2C;Early activation antigen CD69;Activation inducer molecule;AIM;BL-AC/P26;C-type lectin domain family 2 member C;EA1;Early T-cell activation antigen p60;GP32/28;Leukocyte surface antigen Leu-23;MLR-3;CD antigen CD69
- 背景:
- This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. [provided by RefSeq, Aug 2011],
- 功能:
- developmental stage:Earliest inducible cell surface glycoprotein acquired during lymphoid activation.,function:Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.,induction:By antigens, mitogens or activators of PKC on the surface of T and B-lymphocytes. By interaction of IL-2 with the p75 IL-2R on the surface of NK cells.,online information:CD69,PTM:Constitutive Ser/Thr phosphorylation in both mature thymocytes and activated T-lymphocytes.,similarity:Contains 1 C-type lectin domain.,subunit:Homodimer; disulfide-linked.,tissue specificity:Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.,
- 细胞定位:
- Membrane; Single-pass type II membrane protein.
- 组织表达:
- Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells and platelets.
- Western Blot analysis using CD69 Monoclonal Antibody against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded human Tonsil tissues with AEC staining using CD69 Monoclonal Antibody.
- Flow cytometric analysis of Jurkat cells using CD69 Monoclonal Antibody (green) and negative control (purple).