CD247 Monoclonal Antibody(4B10)
- 货号：YM0114
- 应用：WB;IHC;IF;FCM;ELISA
- 种属：Human
- 简介：
- >>Natural killer cell mediated cytotoxicity;>>Th1 and Th2 cell differentiation;>>Th17 cell differentiation;>>T cell receptor signaling pathway;>>Chagas disease;>>Epstein-Barr virus infection;>>Human immunodeficiency virus 1 infection;>>PD-L1 expression and PD-1 checkpoint pathway in cancer
- 蛋白名称：
- T-cell surface glycoprotein CD3 zeta chain
- 免疫原：
- Purified recombinant fragment of human CD247 expressed in E. Coli.
- 特异性：
- CD247 Monoclonal Antibody detects endogenous levels of CD247 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. IF 1:200 - 1:1000. Flow cytometry: 1:200 - 1:400. ELISA: 1:10000. Not yet tested in other applications.
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- CD247;CD3Z;T3Z;TCRZ;T-cell surface glycoprotein CD3 zeta chain;T-cell receptor T3 zeta chain;CD antigen CD247
- 背景：
- The protein encoded by this gene is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
- 功能：
- disease:Defects in CD247 are a cause of primary T-cell immunodeficiency [MIM:610163]. Affected individuals suffer of recurrent infections. Patients T-cell counts are very low and B-cell counts are normal.,domain:The ITAM domains mediate interaction with SHB.,function:Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.,online information:CD247 mutation db,PTM:Phosphorylated on Tyr residues after T-cell receptor triggering.,similarity:Belongs to the CD3Z/FCER1G family.,similarity:Contains 3 ITAM domains.,subunit:The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. CD3-zeta forms either homodimers or heterodimers with CD3-eta. Interacts with SLA and SLA2. Interacts w
- 细胞定位：
- Cell membrane ; Single-pass type I membrane protein.
- 组织表达：
- CD3Z is expressed in normal lymphoid tissue and in peripheral blood mononuclear cells (PBMCs) (PubMed:11722641).
- Western Blot analysis using CD247 Monoclonal Antibody against CD247-hIgGFc transfected HEK293 cell lysate.
- Immunohistochemistry analysis of paraffin-embedded human Thymus tissues with AEC staining using CD247 Monoclonal Antibody.
- Immunofluorescence analysis of K562 cells using CD247 Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye.
- Flow cytometric analysis of Jurkat cells using CD247 Monoclonal Antibody (green) and negative control (purple).
