CCK-4 Monoclonal Antibody
- 货号：YM0098
- 应用：WB;IHC;IF;ELISA
- 种属：Human
- 蛋白名称：
- Inactive tyrosine-protein kinase 7
- 免疫原：
- Purified recombinant fragment of human CCK-4 expressed in E. Coli.
- 特异性：
- CCK-4 Monoclonal Antibody detects endogenous levels of CCK-4 protein.
- 组成：
- Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 稀释：
- WB 1:500 - 1:2000. IHC 1:200 - 1:1000. ELISA: 1:10000.. IF 1:50-200
- 纯化工艺：
- Affinity purification
- 储存：
- -15°C to -25°C/1 year(Do not lower than -25°C)
- 其他名称：
- PTK7;CCK4;Inactive tyrosine-protein kinase 7;Colon carcinoma kinase 4;CCK-4;Protein-tyrosine kinase 7;Pseudo tyrosine kinase receptor 7;Tyrosine-protein kinase-like 7
- 背景：
- This gene encodes a member of the receptor protein tyrosine kinase family of proteins that transduce extracellular signals across the cell membrane. The encoded protein lacks detectable catalytic tyrosine kinase activity, is involved in the Wnt signaling pathway and plays a role in multiple cellular processes including polarity and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012],
- 功能：
- function:May function as a cell adhesion molecule that acts as a regulator of planar cell polarity. Seems to lack tyrosine-protein kinase activity.,similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 7 Ig-like C2-type (immunoglobulin-like) domains.,tissue specificity:Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.,
- 细胞定位：
- Membrane ; Single-pass type I membrane protein . Cell junction . Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
- 组织表达：
- Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
- Western Blot analysis using CCK-4 Monoclonal Antibody against HeLa (1), A431 (2), HCT116 (3), Caco2 (4), HepG2 (5) and MCF-7 (6) cell lysate.
- Immunohistochemistry analysis of paraffin-embedded lung cancer tissues with DAB staining using CCK-4 Monoclonal Antibody.
